myd88 mice  (Jackson Laboratory)

Journal: bioRxiv

Article Title: MyD88 deficiency modestly attenuates disease in a Leigh syndrome mouse model while enrofloxacin accelerates disease

doi: 10.64898/2026.05.13.724988

Figure Lengend Snippet:

Article Snippet: MyD88 (-/-) mice were obtained from the Jackson Lab (strain # 009088) and were originally developed by Hou et al ( ).

Techniques: Produced

Journal: bioRxiv

Article Title: MyD88 deficiency modestly attenuates disease in a Leigh syndrome mouse model while enrofloxacin accelerates disease

doi: 10.64898/2026.05.13.724988

Figure Lengend Snippet: A) NanoString-based analysis of Toll-like receptor ( Tlr ) and MyD88 expression in the brainstem of 45-day old control and Ndufs4 (-/-) mice (see Methods ). Black – Ndufs4 (Ctl), Red – Ndufs4 (-/-) (see Methods ). p-values reflect multiple testing corrected (Holm-Šídák method) pairwise t-tests. *p<0.05, ns and those not shown – not significant. B ) Taqman probe-based qPCR analysis of Tlr7, Tlr9 , and MyD88 expression in 50-day old control and Ndufs4 (-/-) mouse brainstem samples. Normalized to actin, included in each reaction. Black – Ndufs4 (Ctl), Red – Ndufs4 (-/-) (see Methods ). Relative expression calculated using relative standard curve approach. *p<0.05, ns – not significant, by Holm-Šídák method multiple-testing corrected pairwise t-tests with Welch’s correction (no assumption regarding standard deviation).

Article Snippet: MyD88 (-/-) mice were obtained from the Jackson Lab (strain # 009088) and were originally developed by Hou et al ( ).

Techniques: Expressing, Control, Standard Deviation

Journal: bioRxiv

Article Title: MyD88 deficiency modestly attenuates disease in a Leigh syndrome mouse model while enrofloxacin accelerates disease

doi: 10.64898/2026.05.13.724988

Figure Lengend Snippet: A) Impact of enrofloxacin and MyD88 disruption on overall weight trajectory in Nduf4 (-/-) mice (see Results, , ). Replicates (n’s) as indicated. B) Maximum weights of individual animals in A). Replicates (n’s) as indicated in A). ANOVA and pairwise comparisons were not statistically significant. C) Impact of enrofloxacin and MyD88 disruption on the onset of cachexia (weight loss) in Nduf4 (-/-) mice. No curves significantly different by pairwise log-rank comparison. Replicates (n’s) as indicated.

Article Snippet: MyD88 (-/-) mice were obtained from the Jackson Lab (strain # 009088) and were originally developed by Hou et al ( ).

Techniques: Disruption, Comparison

Journal: bioRxiv

Article Title: MyD88 deficiency modestly attenuates disease in a Leigh syndrome mouse model while enrofloxacin accelerates disease

doi: 10.64898/2026.05.13.724988

Figure Lengend Snippet: A) Impact of enrofloxacin and MyD88 disruption on ataxia onset in Nduf4 (-/-) mice. n’s as indicated. The dot (MyD88(-/-) cohort) represents an animal which died prior to presenting with ataxia. P-value shown indicates pairwise log-rank test comparisons between curves indicated - **p<0.005. B) Impact of enrofloxacin and MyD88 disruption on the onset of clasping in Nduf4 (-/-) mice. n’s as indicated. The dot (MyD88(-/-) cohort) represents an animal which died prior to presenting with clasping. P-value shown indicates pairwise log-rank test comparisons between curves indicated - **p<0.005.

Article Snippet: MyD88 (-/-) mice were obtained from the Jackson Lab (strain # 009088) and were originally developed by Hou et al ( ).

Techniques: Disruption

Journal: bioRxiv

Article Title: MyD88 deficiency modestly attenuates disease in a Leigh syndrome mouse model while enrofloxacin accelerates disease

doi: 10.64898/2026.05.13.724988

Figure Lengend Snippet: A) Impact of enrofloxacin and MyD88 disruption on survival in Nduf4 (-/-) mice (see Methods , introduction). P-values shown indicate pairwise log-rank test comparisons between curves indicated - **p=0.0087, *p<0.013. n’s as indicated. B) Cause of death in mice from A). FDIC – found dead in cage, cause of death unknown. C) Summary of findings. Enrofloxacin treatment modestly accelerates disease progression in the Ndufs4 (-/-) model, while loss of MyD88 modestly slows disease progression and extends survival.

Article Snippet: MyD88 (-/-) mice were obtained from the Jackson Lab (strain # 009088) and were originally developed by Hou et al ( ).

Techniques: Disruption, Biomarker Discovery

Journal: NPJ Vaccines

Article Title: IL-1 delineates squalene-based adjuvant efficacy and reactogenicity in a cell-type-specific manner

doi: 10.1038/s41541-026-01420-0

Figure Lengend Snippet: A Study schedule. Mice were injected IM with Ag mixed with A-910823 on days 0 and 14 ( n = 4–10/group), using Myd88 ( B ), Il1r1 ( C ), and Il1rn KO mice ( D ), as well as Myd88 flox/flox (f/f) and Cd11c cre ( E ) or LysM cre mice ( F ). B – F Serum titers of neutralizing antibodies against SARS-CoV-2 on day 28. Bars represent geometric mean titer (GMT); error bars indicate 95% confidence intervals; circles represent neutralizing antibody titers in individual mice. Percentages of T follicular helper (Tfh; PD1 + CXCR5 + ) cells in TCRb + CD4 + cells and percentages of germinal center B (GCB; FAS + GL7 + ) cells in CD19 + cells in draining LNs on day 28. Bars represent means; error bars indicate standard error of the mean; circles represent percentages of Tfh and GCB cells in individual mice. Injection-site swelling scores on day 15. Bars represent means; error bars indicate standard error of the mean; circles represent swelling scores in individual mice. Average body temperature was assessed on Day 12 (08:00–19:59) and on Day 15 (08:00–19:59). Vaccination at day 14 was performed at 18:00. Bars represent means; error bars indicate standard error of the mean. The data were derived from independent experiments: (1) Neutralizing titer/Tfh/GCB measured in the same cohort, (2) Swelling measured in an independent cohort, and (3) Fever measured in an independent cohort. Statistical significance determined using two-tailed Student’s t -test for two-group comparisons or two-way ANOVA. ns, P ≥ 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: Myd88 KO mice, Tlr2 KO mice, Tlr4 KO mice, and Tlr9 KO mice on a C57BL/6 background were obtained from Oriental Bioservice (Kyoto, Japan).

Techniques: Injection, Derivative Assay, Two Tailed Test

Journal: NPJ Vaccines

Article Title: IL-1 delineates squalene-based adjuvant efficacy and reactogenicity in a cell-type-specific manner

doi: 10.1038/s41541-026-01420-0

Figure Lengend Snippet: A Study schedule. Mice were injected IM with Ag mixed with A-910823 on days 0 and 14 ( n = 4–10/group), using Myd88 ( B ), Il1r1 ( C ), and Il1rn KO mice ( D ), as well as Myd88 flox/flox (f/f) and Cd11c cre ( E ) or LysM cre mice ( F ). B – F Serum titers of neutralizing antibodies against SARS-CoV-2 on day 28. Bars represent geometric mean titer (GMT); error bars indicate 95% confidence intervals; circles represent neutralizing antibody titers in individual mice. Percentages of T follicular helper (Tfh; PD1 + CXCR5 + ) cells in TCRb + CD4 + cells and percentages of germinal center B (GCB; FAS + GL7 + ) cells in CD19 + cells in draining LNs on day 28. Bars represent means; error bars indicate standard error of the mean; circles represent percentages of Tfh and GCB cells in individual mice. Injection-site swelling scores on day 15. Bars represent means; error bars indicate standard error of the mean; circles represent swelling scores in individual mice. Average body temperature was assessed on Day 12 (08:00–19:59) and on Day 15 (08:00–19:59). Vaccination at day 14 was performed at 18:00. Bars represent means; error bars indicate standard error of the mean. The data were derived from independent experiments: (1) Neutralizing titer/Tfh/GCB measured in the same cohort, (2) Swelling measured in an independent cohort, and (3) Fever measured in an independent cohort. Statistical significance determined using two-tailed Student’s t -test for two-group comparisons or two-way ANOVA. ns, P ≥ 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: Il1rn KO mice, Myd88 flox mice, Cd11c cre transgenic mice, and LysM cre transgenic mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA).

Techniques: Injection, Derivative Assay, Two Tailed Test

Journal: Cell

Article Title: Distinct components of mRNA vaccines cooperate to instruct efficient germinal center responses

doi: 10.1016/j.cell.2025.11.023

Figure Lengend Snippet: (A) Representative flow cytometry of Tfh cells, GC B cells, and Spike-specific GC B cells (S+) from wild type (WT) and Myd88 −/− mice. (B) Tfh cell, GC B cell, and S+ GC B cell absolute numbers, as detailed in A . (C) Absolute numbers of Tfh cells and GC B cells, as detailed in A , in WT and Tlr7 −/− mice. (D) Frequencies and absolute numbers of Tfh cells and GC B cells, as detailed in A , in mice treated with isotype, anti-IL-1R, or anti-IL-18 mAb. (E) IFN-α levels 8 hours after immunization. Mice were treated with isotype or anti-IL-1R mAb. (F) IFN-α levels in WT and Irf3/7−/− mice 8 hours after immunization. (G) Frequency and absolute numbers of Tfh cells and GC B cells, as detailed in A , in WT and Irf3/7−/− mice. In ( A-G ), mice received 3 μg of Spikevax; n = 6-10 mice per group from 2-3 independent experiments. An unpaired two-tailed Mann-Whitney U test was conducted.

Article Snippet: Myd88 −/− mice: B6.129P2(SJL)- Myd88 tm1.1Defr /J , The Jackson Laboratory , Cat#009088.

Techniques: Flow Cytometry, Two Tailed Test, MANN-WHITNEY

Journal: mBio

Article Title: Induction of myelopoiesis by Candida dubliniensis drives protective trained immunity against sepsis in a Card9-dependent manner

doi: 10.1128/mbio.02906-25

Figure Lengend Snippet: Card9 is required for protection and bone marrow expansion. ( A ) Experimental design: WT and Myd88 −/− or Card9 −/− C57BL/6 mice were immunized IP with C. dubliniensis (1.75 × 10 7 cells in B or 7 × 10 6 cells in C), followed by lethal IP polymicrobial sepsis challenge (1.75 × 10 7 C. albicans in B or 7 × 10 6 C. albicans in C + 8 × 10 7 S. aureus cells) 14 days later. ( B ) Survival of Myd88 −/− mice following lethal sepsis challenge. n = 9–10/group. ( C ) Survival of Card9 −/− mice following lethal sepsis challenge. n = 15–18/group, combined from two experiments. * P < 0.05, ** P < 0.01, log-rank test with Holm-Sidak multiple comparisons test. ( D ) Experimental design: bone marrow from WT and Card9 −/− C57BL/6 mice was isolated 1–2 days post-IP immunization with 1.75 × 10 7 C. dubliniensis cells. ( E ) C. dubliniensis BM infiltration. n = 5–6/group (WT) and n = 11/group ( Card9 −/− , combined from two experiments). ns , WT vs Card9 −/− at either time point, one-way ANOVA with Sidak’s multiple comparisons test. Data are mean ± s.e.m. ( F ) LKS cell expansion. N = 6–7/group, combined from two experiments. ** P < 0.01, **** P < 0.0001, two-way ANOVA with Sidak’s multiple comparison test. Data are expressed as fold change over naïve mice.

Article Snippet: Female C57BL/6 mice (strain #000664), B6.129- Card9 tm1Xlin /J mice (Card9 KO, strain #028652), and B6.129P2(SJL)- Myd88 tm1.1Defr /J mice (Myd88 null, strain #009088), 5–7 weeks of age, were purchased from Jackson Laboratories.

Techniques: Isolation, Comparison